The Milky Way galaxy contains between 200 to 400 billion stars, most with a number of planets in orbit around them. Not all of them are populated or have advanced cultures, however. The notable galactic locations currently a part of the game are located here.
- The Citadel is an ancient deep-space station, presumably constructed by the ancient Prothean species. Since the Prothean extinction, numerous other species have come to call the Citadel home. It serves as the political, cultural, and financial capital of the galactic community. To represent their interests, most species maintain embassies on the Presidium, the Citadel's inner ring.
- Omega is described as "the Terminus Systems' dark, twisted counterpart to the Citadel." Close to the planet Shelba, it is a huge mining station built from the remains of a massive, irregularly shaped asteroid with twisting streets populated with homes, shops and warehouses. Omega's inhabitants are usually lawless. Territory is controlled by the strongest faction in that area and frequently changes hands, often after brutal violence.